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Comparison of different commercial kits showed advantages of the methods based on phenol-chloroform extraction: Total Exosome RNA & Protein Isolation Kit and miRNeasy Serum/Plasma Kit. Here we first examined efficacy of various methods for small RNA isolation from EVs contained in ascitic fluid for subsequent miRNA analysis. The most important factor limiting development of this scientific direction is lack of “gold standards” both for methods of EV isolation from biological fluids and for analyzing their molecular content, including composition of miRNAs. The accumulated pool of data on the role of EVs in carcinogenesis has stimulated investigations of the EV-derived cancer markers. 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In one of his bizarre Q&A-sections possibly mimicking the ‘Ask-me-Anything’ format of Reddit, Tarrant states (2019:17): “ From where did you receive/research/develop your beliefs? The internet, of course. His identity-formation as much as radicalization was driven by the hyper-medial environment in which it was aimed to unfold meaning (Madisson 2016). This is one of the first and key take-aways from the ‘manifesto’ of ‘third position’ radical right ideology terrorist Brenton Tarrant released online (of course – and its content presented extensively below): his understanding of the world and his communication with the world is shaped by the Internet and its specific communication culture. Tarrant thus has much in common with ISIS who painstakingly staged their executions as emblematic hybrid media narratives with the purpose of a maximum of impact (Krona 2019). ![]() Facebook is said to have removed no less than 1,5m videos of the terror attack during the first 24 hours, which demonstrates the magnitude of terrorism as an act of strategic communication (Falkheimer, 2013:44-55) or as Marsh and Mullholland argue: “the attack seemed orchestrated for the social media age” (CNN 2019/1). The far-right terrorist attack of Christchurch was staged ‘in real life’ (IRL) with the purpose to create maximum impact through dissemination online. Moving from idea to action, cognitive and behavioral levels of radicalization (Önnerfors 2018:27). ![]() In the age of online communication and social media, the stage is the same as the Internet and the audience that interactive mix of producers and consumers, the so-called ‘prosumers’ of online ‘user generated content’, where it is difficult to determine the borders between production and consumption of what Bauman has called ‘autotelic violence’ (2017:30). ‘Bühnensucht’ is a German expression denoting an irrational longing to take the stage or as Jenkins put it already in 1974, “terrorism is theater” (Jenkins 1974:7). Terrorism as theater in the age of Internet ![]() ![]() ![]() Ten sweet-styled racers drive their confectionery karts around a taffy track that weaves through the ever-changing environments of the island." "This interactive racer was a breakout hit in American arcades when it was released in 1997. 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Until Ralph's first visit to the game, he had managed to prevent Vanellope from racing for 15 years. However, to stay in power, King Candy made a law stating glitches can't race, because if Vanellope ever did race and cross the finish line, the game would reset. He ruled Sugar Rush after altering the code in which he tried to erase Vanellope's code, made himself king, and Vanellope a glitch. The ruler for most of the movie is King Candy, who is really Turbo in disguise. It is where Ralph also encounters Vanellope Von Schweetz and other numerous characters. It is a kart racing game set on tracks made of candies, cookies, and other sweets. 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